bgx {bgx} | R Documentation |
'bgx' estimates Bayesian Gene eXpression (BGX) measures from an AffyBatch object.
'standalone.bgx' creates various files needed by the bgx standalone binary and places them in a directory. One of these files is 'infile.txt'. In order to run standalone BGX, compile it and run 'bgx <path_to_infile.txt>' from the command line.
bgx(aData, samplesets = NULL, genes = NULL, genesToWatch = NULL, burnin = 8192, iter = 16384, output = c("minimal","trace","all"), probeAff = TRUE, probecat_threshold = 100, adaptive = TRUE, rundir = ".") standalone.bgx(aData, samplesets = NULL, genes = NULL, genesToWatch = NULL, burnin = 8192, iter = 16384, output = c("minimal", "trace", "all"), probeAff = TRUE, probecat_threshold = 100, adaptive = TRUE, batch_size = 50, optimalAR = 0.44, inputdir = "input")
aData |
An AffyBatch object. |
samplesets |
A numeric vector specifying which condition each array belongs to. E.g. if samplesets=c(2,2), then the first two replicates belong to one condition and the last two replicates belong to another condition. If NULL, each array is assumed to belong to a different condition. If the aData object contains information about the experiment design in its phenoData slot, this argument is not required. |
genes |
A numeric vector specifying which genes to analyse. If NULL, all genes are analysed. |
genesToWatch |
A numeric vector specifying which genes to monitor closely amongst those chosen to be analysed (see below for details). |
burnin |
Number of burn-in iterations. |
iter |
Number of post burn-in iterations. |
output |
One of "minimal", "trace" or "all". See below for details. |
probeAff |
Stratify the mean (lambda) of the cross-hybridisation parameter (H) by categories according to probe-level sequence information. |
probecat_threshold |
Minimum amount of probes per probe affinity category. |
adaptive |
Adapt the variance of the proposals for Metropolis Hastings objects (that is: S, H, Lambda, Eta, Sigma and Mu). |
batch_size |
Size of batches for calculating acceptance ratios and updating jumps. |
optimalAR |
Optimal acceptance ratio. |
rundir |
The directory in which to save the output runs. |
inputdir |
The name of the directory in which to place the input files for the standalone binary. |
'bgx' returns an ExpressionSet
object containing gene expression information for each gene under each condition (not each replicate).
'standalone.bgx' returns the path to the BGX input files.
The bgx() method and the bgx standalone binary create a directory in the working directory called 'run.x' (x:1,2,3,...), wherein files are placed for further detailed analysis.
Ernest Turro
Turro, E., Bochkina, N., Hein, A., Richardson, S. (2007) BGX: a Bioconductor package for the Bayesian integrated analysis of Affymetrix GeneChips. BMC Bioinformatics 2007, 8:439.
Hein, A., Richardson, S. (2006) A powerful method for detecting differentially expressed genes from GeneChip arrays that does not require replicates. BMC Bioinformatics 2006, 7:353.
Hein, A., Richardson, S., Causton, H., Ambler, G., Green., P. (2005) BGX: a fully Bayesian integrated approach to the analysis of Affymetrix GeneChip data. Biostatistics, 6, 3, pp. 349-373.
Hekstra, D., Taussig, A. R., Magnasco, M., and Naef, F. (2003) Absolute mRNA concentrations from sequence-specific calibration of oligonucleotide array. Nucleic Acids Research, 31. 1962-1968.
G.O. Roberts, J.S. Rosenthal (September, 2006) Examples of Adaptive MCMC.
# This example requires the 'affydata' and 'hgu95av2cdf' packages if(require(affydata) && require(hgu95av2cdf)) { data(Dilution) eset <- bgx(Dilution, samplesets=c(2,2), probeAff=FALSE, burnin=4096, iter=8192, genes=c(12500:12599), output="all", rundir=file.path(tempdir())) }