plotQA.FCS {rflowcyt} | R Documentation |
Create a scatterplot summaryzing one (or two) parameter(s) for several FCS objects stored in a plate. The points are colored according to their position in the plate (row or column number.)
plotQA.FCS(data,varpos=c(1,2),FUN1=IQR,FUN2=NULL,col="row",nrow=8,ncol=12,ind=c(1:96),labeling=TRUE,...)
data |
a list of fluorescent data from one (or more) FCS object(s) or a cytoset |
varpos |
the numerical column variable position of the data of the FCS object |
FUN1 |
function to summaryze the distribution of the data, e.g. mean, median, IQR, MODE |
FUN2 |
function to summaryze the distribution of the data e.g. mean, median, IQR, MODE |
col |
|
nrow |
numeric, number of rows per plate |
ncol |
numeric, number of columns per plate |
ind |
numeric vector, index of the wells to be plotted |
labeling |
logical, draw plate position (default=TRUE) |
... |
any other arguments are passed to the plot
function |
None.
Nolwenn Le Meur
##Example I: data(flowcyt.data) ##Draw a scatterplot of the median values ##of the Foward scatter and the Side scatter parameters ##of each FCS file. The files correspond to samples store in a 96 well plate. plotQA.FCS(flowcyt.data,varpos=c(1,2),FUN1=median,nrow=8,ncol=10,ind=c(1:80),col="row",pch="*",labeling=FALSE,xlim=c(0,300),ylim=c(0,300),main="FSCmedian vs.SSCmedian by row",xlab="SSC median",ylab="FSC median") ##Example II: ##Draw a a scatterplot of the mode and IQR values for the Foward scatter ##of each FCS file. plotQA.FCS(flowcyt.data,varpos=c(1),FUN1=IQR,FUN2=MODE,nrow=8,ncol=10,ind=c(1:80),col="col",pch="*",labeling=TRUE,xlim=c(0,300),ylim=c(0,300),xlab="FSC mode",ylab="FSC IQR",main="FSC IQR vs.FSC mode by column")